Megakaryocyte development is normal in mice with targeted disruption of Tescalcin

Exp Cell Res. 2012 Mar 10;318(5):662-9. doi: 10.1016/j.yexcr.2012.01.009. Epub 2012 Jan 18.

Abstract

Background: Tescalcin is an EF-hand calcium-binding protein that interacts with the Na+/H+ exchanger 1 (NHE1). Levay and Slepak recently proposed a role for tescalcin in megakaryopoiesis that was independent of NHE1 activity. Their studies using K562 and HEL cell lines, and human CD34+ hematopoietic stem cells suggested an essential role for tescalcin in megakaryocyte differentiation.

Objective: To study the role of tescalcin in megakaryocyte development using a murine model of megakaryopoiesis.

Methods: We generated a mouse with targeted disruption of tescalcin and investigated megakaryocyte development.

Results: Tescalcin-deficient mice had a normal number of megakaryocytes and platelets. The morphology, polyploidization profile, and expression of Fli-1 in bone marrow-derived megakaryocytes were also normal.

Conclusion: Tescalcin does not appear to be necessary for normal megakaryocyte development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Platelets / metabolism
  • Bone Marrow / metabolism
  • Bone Marrow Cells / metabolism
  • Calcium-Binding Proteins / deficiency
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / metabolism
  • Cell Line
  • Female
  • Gene Knockout Techniques
  • Male
  • Megakaryocytes / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Platelet Count
  • Platelet Membrane Glycoprotein IIb / metabolism
  • Polyploidy
  • Thrombopoiesis

Substances

  • Calcium-Binding Proteins
  • Platelet Membrane Glycoprotein IIb
  • Tesc protein, mouse