Development of RNA aptamers for detection of Salmonella Enteritidis

J Microbiol Methods. 2012 Apr;89(1):79-82. doi: 10.1016/j.mimet.2012.01.014. Epub 2012 Jan 28.

Abstract

We developed and evaluated RNA aptamers to analyze their potential for use in detecting Salmonella Enteritidis. The selected aptamer was observed to specifically bind to Salmonella Enteritidis without any cross-reactivity to other Salmonella serovars. Thus, this study suggests that aptamers specific to Salmonella Enteritidis have a high potential for use in presumptive presumptive screening methods or alternative serotyping methods.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / genetics*
  • Bacteriological Techniques / methods*
  • Cross Reactions
  • Humans
  • Mass Screening / methods
  • Salmonella enteritidis / classification*
  • Salmonella enteritidis / genetics
  • Salmonella enteritidis / isolation & purification*
  • Sensitivity and Specificity

Substances

  • Aptamers, Nucleotide