Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking

Angew Chem Int Ed Engl. 2012 May 7;51(19):4618-22. doi: 10.1002/anie.201108695. Epub 2012 Feb 22.

Abstract

This paper describes an approach called ensemble decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood. eDAR has a recovery of over 93% (n=9) with a zero false positive rate (n=8), and provides direct easy access to individual isolated live cells for downstream single-cell manipulation and analysis. We anticipate eDAR will enable new studies of various types of rare cells that circulate in blood.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology
  • Antigens, Neoplasm / immunology
  • Antigens, Neoplasm / metabolism
  • Blood Cells / cytology*
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism
  • Epithelial Cell Adhesion Molecule
  • Flow Cytometry
  • Fluorescent Dyes / chemistry
  • High-Throughput Screening Assays
  • Humans
  • Microscopy, Confocal
  • Neoplastic Cells, Circulating*
  • Neoplastic Stem Cells / cytology
  • Neoplastic Stem Cells / metabolism
  • Receptor, ErbB-2 / immunology
  • Receptor, ErbB-2 / metabolism

Substances

  • Antibodies
  • Antigens, Neoplasm
  • Cell Adhesion Molecules
  • Epithelial Cell Adhesion Molecule
  • Fluorescent Dyes
  • ERBB2 protein, human
  • Receptor, ErbB-2