Objective: To define the sequence type (ST) isolates of Bacillus anthracis by multilocus sequence typing (MLST).
Methods: Fragments of seven housekeeping genes (glpF, gmk, ilvD, pta, pur, pycA, and tpi) were amplified by PCR using the standard primers as described on the website for MLST of Bacillus and the sequences were compared with existing allele sequences on the MLST website.
Results: Two novel allele combinations of the seven loci were found in two isolates 17003-14 and 17003-32.
Conclusion: Two novel ST isolates of B. anthracis were identified by this study and confirmed by the MLST website, and the pubMLST ids were id-1053 and id-1054.