Abstract
As the usage of fluorescence microscopy as a tool to study biological systems continues to grow, so does the need for additional tools that permit the selective detection of proteins of interest. Existing selective and well-characterized kinase inhibitors may be exploited to develop novel small molecule probes useful in imaging kinases by fluorescence microscopy.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Boron Compounds / chemistry
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Cell Cycle
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Cell Cycle Proteins / antagonists & inhibitors*
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Cell Cycle Proteins / metabolism
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Centrosome / enzymology
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Centrosome / ultrastructure
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Chromosome Structures / enzymology
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Chromosome Structures / ultrastructure
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Fluorescent Dyes / chemistry
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HeLa Cells
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Humans
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Microscopy, Fluorescence
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Molecular Imaging / methods*
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Molecular Probes / chemical synthesis
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Polo-Like Kinase 1
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Protein Kinase Inhibitors / chemistry*
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Protein Kinase Inhibitors / pharmacology
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Protein Serine-Threonine Kinases / antagonists & inhibitors*
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Protein Serine-Threonine Kinases / metabolism
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Protein-Tyrosine Kinases / antagonists & inhibitors*
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Protein-Tyrosine Kinases / metabolism
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Proto-Oncogene Proteins / antagonists & inhibitors*
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Proto-Oncogene Proteins / metabolism
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Pteridines / chemistry*
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Pteridines / pharmacology
Substances
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4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
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BI 2536
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Boron Compounds
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Cell Cycle Proteins
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Fluorescent Dyes
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Molecular Probes
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Protein Kinase Inhibitors
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Proto-Oncogene Proteins
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Pteridines
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Protein-Tyrosine Kinases
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Protein Serine-Threonine Kinases
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TTK protein, human