Abstract
We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Babesia / metabolism*
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Babesiosis / diagnosis*
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Cell Adhesion
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Chromatography, Affinity / methods
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Chromatography, Affinity / veterinary*
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Dog Diseases / diagnosis
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Dog Diseases / parasitology
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Dogs
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Protozoan Proteins / chemistry
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Protozoan Proteins / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism*
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Sensitivity and Specificity
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Thrombospondins / chemistry
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Thrombospondins / metabolism*
Substances
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Protozoan Proteins
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Recombinant Proteins
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Thrombospondins