Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni

Vet Parasitol. 2012 Dec 21;190(3-4):595-8. doi: 10.1016/j.vetpar.2012.06.024. Epub 2012 Jun 26.

Abstract

We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Babesia / metabolism*
  • Babesiosis / diagnosis*
  • Cell Adhesion
  • Chromatography, Affinity / methods
  • Chromatography, Affinity / veterinary*
  • Dog Diseases / diagnosis
  • Dog Diseases / parasitology
  • Dogs
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism*
  • Sensitivity and Specificity
  • Thrombospondins / chemistry
  • Thrombospondins / metabolism*

Substances

  • Protozoan Proteins
  • Recombinant Proteins
  • Thrombospondins