Nanoporous silica microparticle interaction with toll-like receptor agonists in macrophages

Acta Biomater. 2012 Dec;8(12):4295-303. doi: 10.1016/j.actbio.2012.07.026. Epub 2012 Jul 27.

Abstract

Nanoporous silica microparticles (NSiO(2)-MP) are considered to be potential drug delivery systems and scaffolding platforms in tissue engineering. However, few biocompatibility studies regarding NSiO(2)-MP interaction with the immune system have been reported. Toll-like receptors (TLR) are involved in host defence as well as autoimmune and inflammatory diseases. The results show that NSiO(2)-MP up to 100μgml(-1) do not affect macrophage cell viability after 24h cell culture. Moreover, NSiO(2)-MP do not compromise the cell viability of TLR-activated Raw 264.7 cells, for either cell surface TLR (TLR1/TLR2/TLR4/TLR6) or endocytic compartment TLR (TLR3/TLR7/TLR9). Furthermore, Raw 264.7 cells do not respond to NSiO(2)-MP exposure in terms of IL-6 or IL-10 secretion. NSiO(2)-MP co-treatment in the presence of TLR ligands does not impair or enhance the secretion of the pro-inflammatory cytokine IL-6 or the regulatory cytokine IL-10. Thus, NSiO(2)-MP do not affect macrophage polarization towards a pro-inflammatory or immunosuppressive status, representing added value in terms of biocompatibility compared with other SiO(2)-based micro- and nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Drug Delivery Systems*
  • Interleukin-10 / immunology
  • Interleukin-10 / metabolism
  • Interleukin-6 / immunology
  • Interleukin-6 / metabolism
  • Ligands
  • Macrophages / cytology
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Mice
  • Nanoparticles*
  • Particle Size
  • Porosity
  • Silicon Dioxide / chemistry
  • Silicon Dioxide / pharmacology*
  • Time Factors
  • Toll-Like Receptors / agonists*
  • Toll-Like Receptors / immunology
  • Toll-Like Receptors / metabolism

Substances

  • IL10 protein, mouse
  • Interleukin-6
  • Ligands
  • Toll-Like Receptors
  • Interleukin-10
  • Silicon Dioxide