Abstract
R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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DNA Damage / genetics
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DNA Replication / genetics*
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DNA, Fungal / chemistry
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DNA, Fungal / metabolism
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Genomic Instability / genetics*
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Meiosis / genetics*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Ribonuclease H / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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Caenorhabditis elegans Proteins
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DNA, Fungal
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HPR1 protein, S cerevisiae
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Nuclear Proteins
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Saccharomyces cerevisiae Proteins
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THO2 protein, S cerevisiae
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THOC-2 protein, C elegans
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Transcription Factors
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Ribonuclease H