Abstract
This study characterized the role of endoplasmic reticulum stress (ERS)-related pathways in arsenic trioxide-induced apoptosis in multidrug-resistant leukemia K562/ADM cells. Arsenic trioxide exposure led to much significant induction of apoptosis in K562/ADM cells than the parental K562 cells, and the chaperone proteins glucose-regulated protein 78, CHOP/GADD153, X-box binding protein-1 and caspase-12 were activated to varying degrees. Furthermore, arsenic trioxide stimulation led to inhibition of P-glycoprotein and Bcl-2 expression. This study demonstrates a missing link between arsenic trioxide and ERS-induced apoptosis, and suggests that the greater effects obtained in drug-resistant K562/ADM cells may be mediated by downregulation of P-glycoprotein and Bcl-2 expression.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
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Antineoplastic Agents / pharmacology*
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Apoptosis / drug effects*
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Arsenic Trioxide
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Arsenicals / pharmacology*
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Caspase 12 / genetics
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Caspase 12 / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Drug Resistance, Multiple
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Drug Resistance, Neoplasm
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Endoplasmic Reticulum / drug effects*
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Endoplasmic Reticulum / genetics
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Endoplasmic Reticulum / metabolism
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Endoplasmic Reticulum Chaperone BiP
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Endoplasmic Reticulum Stress / drug effects*
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Endoplasmic Reticulum Stress / genetics
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Gene Expression Regulation, Neoplastic
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Growth Inhibitors / pharmacology*
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism
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Humans
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K562 Cells
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Oxides / pharmacology*
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Regulatory Factor X Transcription Factors
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Signal Transduction
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Transcription Factor CHOP / genetics
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Transcription Factor CHOP / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antineoplastic Agents
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Arsenicals
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DDIT3 protein, human
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DNA-Binding Proteins
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Endoplasmic Reticulum Chaperone BiP
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Growth Inhibitors
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Heat-Shock Proteins
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Oxides
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Proto-Oncogene Proteins c-bcl-2
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Regulatory Factor X Transcription Factors
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Transcription Factors
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Transcription Factor CHOP
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Caspase 12
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Arsenic Trioxide