Effects of okadaic acid on haemocytes from Mytilus galloprovincialis: a comparison between field and laboratory studies

Maria Prado-Alvarez; Fernanda Flórez-Barrós; Alexia Sexto-Iglesias; Josefina Méndez; Juan Fernandez-Tajes

doi:10.1016/j.marenvres.2012.08.011

Effects of okadaic acid on haemocytes from Mytilus galloprovincialis: a comparison between field and laboratory studies

Mar Environ Res. 2012 Oct:81:90-3. doi: 10.1016/j.marenvres.2012.08.011. Epub 2012 Sep 7.

Authors

Maria Prado-Alvarez¹, Fernanda Flórez-Barrós, Alexia Sexto-Iglesias, Josefina Méndez, Juan Fernandez-Tajes

Affiliation

¹ Department of Cell and Molecular Biology, Campus A Zapateira s/n, University of A Coruña, A Coruña, Spain.

PMID: 23000349
DOI: 10.1016/j.marenvres.2012.08.011

Abstract

Individuals of Mytilus galloprovincialis, contaminated with Diarrheic Shellfish Poisoning (DSP) toxins, were studied with the aim to correlate the okadaic acid (OA) body burden and the percentage of damaged haemocytes by quantifying annexin V positive cells by flow cytometry. Results showed less percentage of damaged haemocytes in high OA contaminated samples. These data were compared with results of in vitro assays of mussel haemocytes exposed to increased concentrations of OA. Similarly, haemocytes exposed to the most concentrated OA solution were less damaged.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Annexin A5 / analysis
Annexin A5 / pharmacology
Enzyme Inhibitors / pharmacology
Hemocytes / drug effects
Marine Toxins / analysis
Marine Toxins / metabolism
Mytilus / drug effects*
Okadaic Acid / pharmacology*
Research Design / standards

Substances

Annexin A5
Enzyme Inhibitors
Marine Toxins
Okadaic Acid