Visualization of the intracellular location and stability of DNA origami with a label-free fluorescent probe

Chem Commun (Camb). 2012 Nov 28;48(92):11301-3. doi: 10.1039/c2cc36185j.

Abstract

We report a label-free fluorescent strategy to study the distribution and stability of DNA origami nanostructures in live, cellular systems, using carbazole-based biscyanine as a probe molecule which has the characteristic property of restriction of intramolecular rotation (RIR) induced emission.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbocyanines / chemistry
  • DNA / chemistry*
  • Fluorescent Dyes / chemistry*
  • Humans
  • MCF-7 Cells
  • Microscopy, Fluorescence
  • Nanostructures / chemistry

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • cyanine dye 3
  • cyanine dye 5
  • DNA