Effect of TiO2 scaffolds coated with alginate hydrogel containing a proline-rich peptide on osteoblast growth and differentiation in vitro

J Biomed Mater Res A. 2013 Jun;101(6):1768-77. doi: 10.1002/jbm.a.34458. Epub 2012 Nov 30.

Abstract

The aim of this study was to investigate the effect of TiO2 scaffold (SC) coated with an alginate hydrogel containing a proline-rich peptide (P2) on osteoblast proliferation and differentiation in vitro. Peptide release was evaluated and a burst release was observed during the first hours of incubation, and then progressively released overtime. No changes were observed in the cytotoxicity after 48 h of seeding MC3T3-E1 cells on the coated and uncoated TiO2 SC. The amount of cells after 7 days was higher on uncoated TiO2 SC than on alginate-coated TiO2 SC, measured by DNA content and scanning electron microscope imaging. In addition, while lower expression of integrin beta1 was detected for alginate-coated TiO2 SC at this time point, similar gene expression was observed for other integrins, fibronectin-1, and several osteoblast differentiation markers. After 21 days, gene expression of integrin beta3, fibronectin-1, osterix, and collagen-I was increased in alginate-coated compared to TiO2 SC. Moreover, increased gene expression of integrin alpha8, bone morphogenetic protein 2, interleukin-6, and collagen-I was found on P2 alginate-coated TiO2 SC compared to alginate-coated TiO2 SC. In conclusion, our results indicate that alginate-coated TiO2 SC can act as a matrix for delivery of proline-rich peptides increasing osteoblast differentiation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / pharmacology*
  • Amino Acid Sequence
  • Animals
  • Biomarkers / metabolism
  • Cell Adhesion / drug effects
  • Cell Adhesion / genetics
  • Cell Count
  • Cell Differentiation / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Coated Materials, Biocompatible / pharmacology*
  • Culture Media
  • Gene Expression Regulation / drug effects
  • Glucuronic Acid / pharmacology
  • Hexuronic Acids / pharmacology
  • Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology*
  • L-Lactate Dehydrogenase / metabolism
  • Mice
  • Molecular Sequence Data
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects
  • Osteoblasts / metabolism
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Proline-Rich Protein Domains
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tissue Scaffolds / chemistry*
  • Titanium / pharmacology*

Substances

  • Alginates
  • Biomarkers
  • Coated Materials, Biocompatible
  • Culture Media
  • Hexuronic Acids
  • Peptides
  • RNA, Messenger
  • titanium dioxide
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Glucuronic Acid
  • Titanium
  • L-Lactate Dehydrogenase