Premise of the study: Microsatellite makers were developed in Kleinia neriifolia, an endemic Asteraceae species on the Canary Islands, for investigation of the population genetic structure and colonization history of this insular species. •
Methods and results: Seventeen polymorphic and seven monomorphic microsatellite loci were isolated and characterized from K. neriifolia using the protocol of Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO). At the population level, the number of alleles detected per locus varied from two to 16, and the observed and expected heterozygosities were 0.105-1.000 and 0.100-0.923, respectively. •
Conclusions: These highly informative loci are potentially useful to obtain a deeper understanding of the evolutionary process of K. neriifolia.