The aim of this study was to evaluate pain treatment with morphine administered by emergency medical service personnel (EMSP) to patients with chest pain and patients with pain in extremities because of trauma. This is a retrospective chart review of 2021 patients with chest pain and 887 patients with trauma. Pain was assessed using a 0-10 Numerical Rating Scale, and measured at the beginning and at the end of the ambulance care period. Trauma patients experienced more pain both at the start and at the end of the treatment than patients with chest pain [median 8 (interquartile ranges (IQR 6-9)) vs. 6 (IQR 4-7) and 4 (IQR 2-6) vs. 2 (IQR 0-4), P<0.001], but were treated with similar doses as in patients with chest pain [median 7.5 (IQR 5-10) and 5 (IQR 2.5-7.5), P=0.09]. Inadequate analgesia was frequently observed for both patient groups. The protocol was not fully utilized, suggesting that education in pharmacology and follow-up of the EMSP is required.