Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

Anal Biochem. 2013 Sep 1;440(1):18-22. doi: 10.1016/j.ab.2013.04.026. Epub 2013 May 7.

Abstract

We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maize chlorotic mottle virus (MCMV). 11-Mercaptoundecanoic acid was applied on a gold surface to form a self-assembled monolayer, and a layer of anti-MCMV antibody was crosslinked on the surface for specific recognition of MCMV. The effects of coupling reaction time and antibody concentration on detection sensitivity were studied. The coverage mass change is a function of the concentration of MCMV with a dynamic range from 1 to 1000 ppb. The detection limit is approximately 1 ppb, which is approximately two orders of magnitude higher than that of the existing enzyme-linked immunosorbent assay (ELISA) method. The developed SPR sensor showed highly specific recognition for both purified MCMV and crude extracts from real-world samples.

Keywords: Biosensor; Maize chlorotic mottle virus; Surface plasmon resonance; Virus detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Enzyme-Linked Immunosorbent Assay
  • Fatty Acids / analysis*
  • Sensitivity and Specificity
  • Sulfhydryl Compounds / analysis*
  • Surface Plasmon Resonance / methods*
  • Tombusviridae / isolation & purification*
  • Zea mays / virology*

Substances

  • 11-mercaptoundecanoic acid
  • Fatty Acids
  • Sulfhydryl Compounds