Evaluation of the differentiation status of single embryonic stem cells using scanning electrochemical microscopy

Yoshiharu Matsumae; Toshiharu Arai; Yasufumi Takahashi; Kosuke Ino; Hitoshi Shiku; Tomokazu Matsue

doi:10.1039/c3cc43126f

Evaluation of the differentiation status of single embryonic stem cells using scanning electrochemical microscopy

Chem Commun (Camb). 2013 Jul 25;49(58):6498-500. doi: 10.1039/c3cc43126f. Epub 2013 Jun 12.

Authors

Yoshiharu Matsumae¹, Toshiharu Arai, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Affiliation

¹ Graduate School of Environmental Studies, Tohoku University, Aramaki 6-6-11-605, Aoba, Sendai 980-8579, Japan.

PMID: 23760226
DOI: 10.1039/c3cc43126f

Abstract

The differentiation status of single live embryonic stem (ES) cells was quantitatively evaluated by monitoring the activity of alkaline phosphatase (ALP), an undifferentiation marker of ES cells, using scanning electrochemical microscopy (SECM).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / metabolism*
Animals
Cell Differentiation
Cells, Cultured
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / metabolism*
Mice
Microscopy, Electrochemical, Scanning
Single-Cell Analysis / methods*
Tretinoin / pharmacology

Substances

Tretinoin
Alkaline Phosphatase