Abstract
A synthetic glycoarray containing non-reducing α-galactopyranosyl moieties related to mucin O-glycans of the parasite Trypanosoma cruzi was evaluated by a chemiluminescent enzyme-linked immunosorbent assay with sera from patients with chronic Chagas disease. Our data revealed the disaccharide Galα(1,3)Galβ as the immunodominant glycotope, which may eventually be employed as a diagnostic antigen for Chagas disease.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antigen-Antibody Reactions
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Carbohydrate Conformation
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Chagas Disease / diagnosis*
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Chagas Disease / immunology
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Chagas Disease / microbiology
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Enzyme-Linked Immunosorbent Assay
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Epitopes / chemistry*
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Epitopes / immunology
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Galactose / chemistry*
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Galactose / immunology
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Humans
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Trypanosoma cruzi / chemistry*
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Trypanosoma cruzi / immunology