The force of transporting a single amino acid into the living cell measured using atomic force microscopy

Xin Shang; Yuping Shan; Yangang Pan; Mingjun Cai; Junguang Jiang; Hongda Wang

doi:10.1039/c3cc43779e

The force of transporting a single amino acid into the living cell measured using atomic force microscopy

Chem Commun (Camb). 2013 Sep 25;49(74):8163-5. doi: 10.1039/c3cc43779e.

Authors

Xin Shang¹, Yuping Shan, Yangang Pan, Mingjun Cai, Junguang Jiang, Hongda Wang

Affiliation

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.

PMID: 23925403
DOI: 10.1039/c3cc43779e

Abstract

We used single molecule force spectroscopy (SMFS) to investigate the interacting force between single cysteine and amino acid transporters in eukaryotic cell membranes. We measured the transporting force of cysteine and found that its conformation on the AFM tip is important for discriminating the substrate in the transporting pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / metabolism*
Cell Survival
Eukaryotic Cells / metabolism*
Eukaryotic Cells / ultrastructure*
HeLa Cells
Humans
Microscopy, Atomic Force
Molecular Structure

Substances

Amino Acids