Pyranonigrin E: a PKS-NRPS hybrid metabolite from Aspergillus niger identified by genome mining

Chembiochem. 2013 Nov 4;14(16):2095-9. doi: 10.1002/cbic.201300430. Epub 2013 Sep 17.

Abstract

Induced production of PKS-NRPS metabolites: the genome mining approach is useful for obtaining new compounds. We activated a dormant PKS-NRPS gene cluster in Aspergillus niger ATCC 1015 by expressing its dedicated transcriptional activator (PynR). As a result, the transformant expressing pynR produced a new pyranonigrin compound that we have named pyranonigirin E.

Keywords: gene expression; genome mining; peptides; polyketides; transcriptional factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus niger / chemistry
  • Aspergillus niger / genetics
  • Aspergillus niger / metabolism*
  • Data Mining*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Genome, Fungal*
  • Magnetic Resonance Spectroscopy
  • Molecular Conformation
  • Multigene Family
  • Peptide Synthases / genetics*
  • Peptide Synthases / metabolism
  • Polyketide Synthases / genetics*
  • Polyketide Synthases / metabolism
  • Pyrones / chemistry
  • Pyrones / metabolism*
  • Pyrroles / chemistry
  • Pyrroles / metabolism*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism

Substances

  • Fungal Proteins
  • Pyrones
  • Pyrroles
  • Trans-Activators
  • pyranonigrin E
  • Polyketide Synthases
  • Peptide Synthases
  • non-ribosomal peptide synthase