Abstract
Induced production of PKS-NRPS metabolites: the genome mining approach is useful for obtaining new compounds. We activated a dormant PKS-NRPS gene cluster in Aspergillus niger ATCC 1015 by expressing its dedicated transcriptional activator (PynR). As a result, the transformant expressing pynR produced a new pyranonigrin compound that we have named pyranonigirin E.
Keywords:
gene expression; genome mining; peptides; polyketides; transcriptional factors.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aspergillus niger / chemistry
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Aspergillus niger / genetics
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Aspergillus niger / metabolism*
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Data Mining*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Genetic Vectors / genetics
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Genetic Vectors / metabolism
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Genome, Fungal*
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Magnetic Resonance Spectroscopy
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Molecular Conformation
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Multigene Family
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Peptide Synthases / genetics*
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Peptide Synthases / metabolism
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Polyketide Synthases / genetics*
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Polyketide Synthases / metabolism
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Pyrones / chemistry
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Pyrones / metabolism*
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Pyrroles / chemistry
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Pyrroles / metabolism*
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Trans-Activators / genetics
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Trans-Activators / metabolism
Substances
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Fungal Proteins
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Pyrones
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Pyrroles
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Trans-Activators
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pyranonigrin E
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Polyketide Synthases
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Peptide Synthases
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non-ribosomal peptide synthase