Objective: To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine.
Design and methods: HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles.
Results: The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4 nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%.
Conclusions: The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer.
Keywords: Bladder carcinoma; Gold nanoparticles; Hepatoma upregulated protein; Magnetic nanoparticles; Squamous cell carcinoma; Transitional cell carcinoma; Urinary biomarkers.
© 2013.