Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis

Clin Biochem. 2014 Jan;47(1-2):104-10. doi: 10.1016/j.clinbiochem.2013.10.022. Epub 2013 Oct 29.

Abstract

Objective: To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine.

Design and methods: HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles.

Results: The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4 nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%.

Conclusions: The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer.

Keywords: Bladder carcinoma; Gold nanoparticles; Hepatoma upregulated protein; Magnetic nanoparticles; Squamous cell carcinoma; Transitional cell carcinoma; Urinary biomarkers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Female
  • Gold / chemistry*
  • Humans
  • Limit of Detection
  • Male
  • Metal Nanoparticles*
  • Middle Aged
  • Neoplasm Proteins / genetics*
  • Polymerase Chain Reaction
  • RNA / urine*
  • Urinary Bladder Neoplasms / diagnosis*

Substances

  • DLGAP5 protein, human
  • Neoplasm Proteins
  • RNA
  • Gold