Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

Chi-Jung Wu; Ya-Ping Chen; Hsuan-Chen Wang; Ih-Jen Su; Wen-Chien Ko; Jiann-Shiuh Chen; Chao-Neng Cheng; Nan-Yao Lee; H Sunny Sun; Chia-Yu Chi; Tsai-Yun Chen

doi:10.1016/j.diagmicrobio.2013.08.007

Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

Diagn Microbiol Infect Dis. 2014 Feb;78(2):141-3. doi: 10.1016/j.diagmicrobio.2013.08.007. Epub 2013 Nov 20.

Authors

Affiliations

¹ National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan; Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan. Electronic address: wucj@nhri.org.tw.
² Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
³ National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan.
⁴ Department of Pediatrics, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
⁵ Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁶ National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan; Department of Pediatrics, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan.
⁷ Department of Internal Medicine, National Cheng Kung University Hospital and Medical College, Tainan, Taiwan. Electronic address: teresa@mail.ncku.edu.tw.

PMID: 24268535
DOI: 10.1016/j.diagmicrobio.2013.08.007

Abstract

Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.

Keywords: Electrospray ionization mass spectrometry; Fungal infection; PCR; Paraffin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Child
Child, Preschool
Fungi / classification*
Fungi / genetics*
Humans
Middle Aged
Multilocus Sequence Typing*
Mycoses / diagnosis
Mycoses / microbiology
Polymerase Chain Reaction*
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization*
Young Adult