I-DNASE21 system: development and SWGDAM validation of a new STR 21-plex reaction

Forensic Sci Int Genet. 2014 Jan;8(1):10-9. doi: 10.1016/j.fsigen.2013.06.014. Epub 2013 Sep 7.

Abstract

I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction. This system has been designed to analyze all the STR loci included in the Combined DNA Index System (CODIS), Interpol Standard Set of Loci (ISSL), Extended European Standard Set (ESS-Extended), UK National Criminal Intelligence DNA Database (NDNAD) and German Core loci (GCL). This manuscript presents the validation of the I-DNASE21 system according to the revised guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). The results of this validation, added to the extremely high discriminatory power showed, suggest that I-DNASE21 could be a potentially helpful tool for identification and kinship determination even in complex paternity cases.

Keywords: DNA database; DNA typing; Forensic; Kinship; Multiplex; STR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Databases, Genetic
  • Deoxyribonucleases / genetics*
  • Heterozygote
  • Humans
  • Microsatellite Repeats*
  • Polymerase Chain Reaction
  • Reproducibility of Results
  • Species Specificity

Substances

  • Deoxyribonucleases