[Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene]

N E Broude; G S Monastyrskaia; K E Petrukhin; A V Grishin; N I Kiiatkin

[Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene]

Bioorg Khim. 1987 Jan;13(1):14-9.

[Article in Russian]

Authors

N E Broude, G S Monastyrskaia, K E Petrukhin, A V Grishin, N I Kiiatkin

PMID: 2436627

Abstract

mRNA coding for beta-subunit of Na+, K+-ATPase from pig kidney is about 24-25S as deduced from the hybridization pattern of poly (A)+-RNA with two synthetic oligonucleotides structurally corresponding to two peptides isolated from the tryptic hydrolyzate of beta-subunit. Cloning of cDNA allowed to determine the complete structure of the gene and to deduce the amino acid sequence of beta-subunit of Na+, K+-ATPase. The beta-subunit contains 302 amino acid residues and the protein is not processed at the N-nor at the C-terminus.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
DNA / genetics
Genes*
Hydrolysis
Kidney / enzymology*
Nucleic Acid Hybridization
RNA, Messenger / genetics*
RNA-Directed DNA Polymerase / genetics*
Sodium-Potassium-Exchanging ATPase / genetics*
Swine
Templates, Genetic
Trypsin

Substances

RNA, Messenger
DNA
RNA-Directed DNA Polymerase
Trypsin
Sodium-Potassium-Exchanging ATPase