Abstract
We have identified a nuclear protein which binds specifically to the first intron of rat alpha 2-macroglobulin gene. The protein became insoluble at low salt concentration retaining the binding specificity. Its molecular weight was estimated to be 22 kilodalton by a protein blotting procedure. The binding site of the protein determined by DNase I footprinting was an AT-strech which shared 80% homology with the cleavage consensus of Drosophila topoisomerase II.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cell Nucleus / analysis*
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DNA / metabolism
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DNA Restriction Enzymes
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DNA-Binding Proteins / metabolism*
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Deoxyribonuclease I
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Introns*
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Liver / ultrastructure*
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Molecular Weight
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Plasmids
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Promoter Regions, Genetic
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Rats
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Repetitive Sequences, Nucleic Acid
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Solubility
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alpha-Macroglobulins / genetics*
Substances
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DNA-Binding Proteins
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alpha-Macroglobulins
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DNA
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DNA Restriction Enzymes
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Deoxyribonuclease I