Systemic and local inflammatory response in women with preterm prelabor rupture of membranes

PLoS One. 2014 Jan 21;9(1):e85277. doi: 10.1371/journal.pone.0085277. eCollection 2014.

Abstract

Objective: To evaluate the inflammatory pattern in maternal circulation, amniotic cavity, cervix and vagina from women with preterm prelabor rupture of membranes (PPROM) considering the occurrence of microbial invasion of the amniotic cavity (MIAC).

Methodology: A prospective study was performed in 58 women with PPROM before 34+0 weeks of gestational age. Twenty-six proteins were analyzed by a multiple immunoassay in samples of amniotic fluid, serum, cervix and vagina. Association of an inflammatory response in the invasive and non-invasive samples with MIAC was investigated.

Results: The rate of MIAC was 36.2% (21/58). Both amniotic fluid IL-6 and cervical C-reactive protein (CRP) showed to be independent predictors of MIAC. A cut-off level of cervical CRP≥1836 pg/mL showed a detection rate of 75%, false positive rate of 19% and positive and negative predictive values to predict MIAC of 67% and 87%, respectively. There were no independent biomarkers of MIAC either in the serum or vaginal compartment.

Conclusion: A cervical inflammatory response mediated by CRP was observed in PPROM women with MIAC. Evaluation of serum or vaginal samples did not add valuable information regarding the outcome evaluated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amniotic Fluid / chemistry
  • Amniotic Fluid / microbiology*
  • C-Reactive Protein / metabolism
  • Cervix Uteri / chemistry
  • Cervix Uteri / microbiology
  • Female
  • Fetal Membranes, Premature Rupture / blood
  • Fetal Membranes, Premature Rupture / microbiology*
  • Gestational Age
  • Gram-Negative Bacterial Infections / blood
  • Gram-Negative Bacterial Infections / microbiology*
  • Gram-Positive Bacterial Infections / blood
  • Gram-Positive Bacterial Infections / microbiology*
  • Humans
  • Infant, Newborn
  • Inflammation / blood
  • Inflammation / microbiology
  • Interleukin-6 / blood
  • Mycoses / blood
  • Mycoses / microbiology*
  • Obstetric Labor, Premature / blood
  • Obstetric Labor, Premature / microbiology*
  • Pregnancy
  • Prospective Studies
  • Vagina / chemistry
  • Vagina / microbiology

Substances

  • IL6 protein, human
  • Interleukin-6
  • C-Reactive Protein

Grants and funding

This work was supported by a Rio Hortega grant from Instituto de Salud Carlos III in Spain (CM09/00213) and grants from Spanish government (FIS PI10/01308). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.