Aim: In this study, Ce(3+)-doped lanthanum(III) fluoride (LaF3:Ce(3+)) nanoparticles were synthesized by a wet-chemistry method in dimethyl sulfoxide (DMSO) and their application as an intracellular light source for photodynamic activation was demonstrated.
Materials & methods: The LaF3:Ce(3+)/DMSO nanoparticles have a strong green emission with a peak at approximately 520 nm, which is effectively overlapped with the absorption of protoporphyrin IX (PPIX). The nanoparticles were encapsulated into poly(D,L-lactide-co-glycolide (PLGA) microspheres along with PPIX. Upon irradiation with x-rays (90 kV), energy transfer from the LaF3:Ce(3+)/DMSO nanoparticles to PPIX occurs and singlet oxygen is generated for cancer cell damage.
Results: The LaF3:Ce(3+)/DMSO/PLGA or LaF3:Ce(3+)/DMSO/PPIX/PLGA microspheres alone caused only sublethal cytotoxicity to the cancer cells. Upon x-ray irradiation, the LaF3:Ce(3+)/DMSO/PPIX/PLGA microspheres induced oxidative stress, mitochondrial damage and DNA fragmentation on prostate cancer cells (PC3).
Discussion: The results indicate that x-rays can activate LaF3:Ce(3+) and PPIX nanocomposites, which can be a novel method for cancer destruction.
Keywords: LaF3:Ce3+; cancer; energy transfer; luminescence; nanoparticle; photodynamic therapy; photosensitizer; prostate cancer; singlet oxygen; treatment.