Expression in bacteria of beta-galactosidase fusion proteins carrying antigenic determinants of the two X gene products of bovine leukemia virus

Leukemia. 1988 Jan;2(1):1-5.

Abstract

A cDNA corresponding to the bovine leukemia virus post-envelope region (X gene) was subcloned into the lambda gt11 expression vector. Two large protein fragments corresponding respectively to the long and short open reading frames of the X region were expressed as beta-galactosidase fusion proteins. These products were specifically recognized by sera from bovine leukemia virus-infected cattle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle / blood
  • Cell Line
  • Epitopes
  • Escherichia coli / enzymology*
  • Galactosidases / physiology*
  • Leukemia Virus, Bovine / genetics*
  • Molecular Weight
  • Protein Biosynthesis
  • Retroviridae / genetics*
  • Viral Fusion Proteins / immunology*
  • beta-Galactosidase / physiology*

Substances

  • Epitopes
  • Viral Fusion Proteins
  • Galactosidases
  • beta-Galactosidase