Abstract
A cDNA corresponding to the bovine leukemia virus post-envelope region (X gene) was subcloned into the lambda gt11 expression vector. Two large protein fragments corresponding respectively to the long and short open reading frames of the X region were expressed as beta-galactosidase fusion proteins. These products were specifically recognized by sera from bovine leukemia virus-infected cattle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle / blood
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Cell Line
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Epitopes
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Escherichia coli / enzymology*
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Galactosidases / physiology*
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Leukemia Virus, Bovine / genetics*
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Molecular Weight
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Protein Biosynthesis
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Retroviridae / genetics*
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Viral Fusion Proteins / immunology*
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beta-Galactosidase / physiology*
Substances
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Epitopes
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Viral Fusion Proteins
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Galactosidases
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beta-Galactosidase