Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry

MingWei Yang; WeiHua Wu; YaJuan Ruan; LiMei Huang; Zujian Wu; Yong Cai; FengFu Fu

doi:10.1016/j.aca.2014.01.003

Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry

Anal Chim Acta. 2014 Feb 17:812:12-7. doi: 10.1016/j.aca.2014.01.003. Epub 2014 Jan 10.

Authors

MingWei Yang¹, WeiHua Wu², YaJuan Ruan², LiMei Huang², Zujian Wu³, Yong Cai⁴, FengFu Fu⁵

Affiliations

¹ Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China; Department of Chemistry, Florida International University, Miami, FL 33199, USA.
² Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China.
³ Department of Plant Protection, Fujian Agriculture and Forest University, Fuzhou 350002, Fujian, China.
⁴ Department of Chemistry, Florida International University, Miami, FL 33199, USA.
⁵ Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China; Department of Plant Protection, Fujian Agriculture and Forest University, Fuzhou 350002, Fujian, China. Electronic address: fengfu@fzu.edu.cn.

PMID: 24491758
DOI: 10.1016/j.aca.2014.01.003

Abstract

In this study, an ultra-sensitive method for the quantification of lysozyme based on the Gd(3+) diethylenetriamine-N,N,N',N″,N″-pentaacetic acid labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) was described. The Gd(3+)-tagged lysozyme was effectively separated by capillary electrophoresis (CE) and sensitively determined by inductively coupled plasma mass spectrometry (ICP-MS). Based on the gadolinium-tagging and CE-ICP-MS, the lysozyme was determined within 12 min with an extremely low detection limit of 3.89 attomole (3.89×10(-11) mol L(-1) for 100 nL of sample injection) and a RSD<6% (n=5). The proposed method has been successfully used to detect lysozyme in saliva samples with a recovery of 91-106%, suggesting that our method is sensitive and reliable. The success of the present method provides a new potential for the biological assays and sensitive detection of low-abundant proteins.

Keywords: Capillary electrophoresis; Inductively coupled plasma mass spectrometry; Lysozyme; Protein; Saliva.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chelating Agents / chemistry*
Electrophoresis, Capillary / methods*
Gadolinium / chemistry
Limit of Detection
Mass Spectrometry / methods*
Muramidase / analysis*

Substances

Chelating Agents
Gadolinium
Muramidase