Genome-wide mRNA and miRNA analysis of peripheral blood mononuclear cells (PBMC) reveals different miRNAs regulating HIV/HCV co-infection

P Gupta; B Liu; J Q Wu; V Soriano; E Vispo; A P Carroll; B J Goldie; M J Cairns; N K Saksena

doi:10.1016/j.virol.2013.12.026

Genome-wide mRNA and miRNA analysis of peripheral blood mononuclear cells (PBMC) reveals different miRNAs regulating HIV/HCV co-infection

Virology. 2014 Feb:450-451:336-49. doi: 10.1016/j.virol.2013.12.026. Epub 2014 Jan 14.

Authors

P Gupta¹, B Liu², J Q Wu³, V Soriano⁴, E Vispo⁴, A P Carroll⁵, B J Goldie³, M J Cairns⁶, N K Saksena⁷

Affiliations

¹ Retroviral Genetics Division, Centre for Virus Research, Westmead Millennium Institute and Westmead Hospital, The University of Sydney, Westmead, NSW 2145, Australia.
² School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia. Electronic address: BLiu@ccia.unsw.edu.au.
³ School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia; Schizophrenia Research Institute, 405 Liverpool St, Darlinghurst, NSW 2010, Australia.
⁴ Department of Infectious Diseases, Hospital Carlos III, Sinesio Delgado 10, 28029 Madrid, Spain.
⁵ School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia.
⁶ School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, Hunter Medical Research Institute, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia; Schizophrenia Research Institute, 405 Liverpool St, Darlinghurst, NSW 2010, Australia. Electronic address: Murray.Cairns@newcastle.edu.au.
⁷ Retroviral Genetics Division, Centre for Virus Research, Westmead Millennium Institute and Westmead Hospital, The University of Sydney, Westmead, NSW 2145, Australia. Electronic address: nitin.saksena@sydney.edu.au.

PMID: 24503097
DOI: 10.1016/j.virol.2013.12.026

Abstract

Co-infection with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) is common due to shared transmission routes. The genomic basis of HIV/HCV co-infection and its regulation by microRNA (miRNA) is unknown. Therefore, our objective was to investigate genome-wide mRNA expression and its regulation by miRNA in primary PBMCs derived from 27 patients (5 HCV - mono-infected, 5 HIV-mono-infected, 12 HCV/HIV co-infected, and 5 healthy controls). This revealed 27 miRNAs and 476 mRNAs as differentially expressed (DE) in HCV/HIV co-infection when compared to controls (adj p<0.05). Our study shows the first evidence of miRNAs specific for co-infection, several of which are correlated with key gene targets demonstrating functional relationships to pathways in cancer, immune-function, and metabolism. Notable was the up regulation of HCV-specific miR-122 in co-infection (FC>50, p=4.02E-06), which may have clinical/biological implications.

Keywords: Co-infection; HCV; HIV; Microarray; miRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Case-Control Studies
Coinfection / genetics*
Coinfection / metabolism
Coinfection / virology
Female
Gene Expression Profiling
Genome
HIV Infections / genetics*
HIV Infections / metabolism
HIV Infections / virology
HIV-1 / genetics
HIV-1 / physiology*
Hepacivirus / genetics
Hepacivirus / physiology*
Hepatitis C / genetics*
Hepatitis C / metabolism
Hepatitis C / virology
Humans
Leukocytes, Mononuclear / metabolism*
Male
MicroRNAs / genetics*
MicroRNAs / metabolism
Middle Aged

Substances

MicroRNAs