Changes in serum cytokines in response to musculoskeletal surgical trauma

BMC Res Notes. 2014 Mar 7:7:128. doi: 10.1186/1756-0500-7-128.

Abstract

Background: Trauma induces local and subsequent systemic inflammatory reactions, and when the cytokine production is deregulated, a systemic inflammatory response syndrome with a potentially lethal outcome can occur. The understanding of the physiological mechanism of the cytokine network would be useful to better comprehend pathological conditions.

Methods: We analysed a panel of 30 cytokines in the serum of 20 patients operated with total hip replacement. Cytokine release was assessed postoperatively up to 6 days by a multiplex antibody bead kit and compared to pre-operative values.

Results: Surgery induced significant increments in serum levels of IL-2R at 6 days after surgery, in levels of IL-6 at 6 hours after surgery and at 1 day after surgery, in levels of IL-8 at 6 hours after surgery, in levels of IL-16 at 6 hours and at 1 day after surgery. Significant decreases in serum levels of IL-1Rα were found at the end of surgery, in levels of IL-12 at the end of surgery and at 6 hours after, and in levels of Eotaxin during all phases of the postoperative course.

Conclusions: The major findings were significant increases in systemic levels of the pro-inflammatory cytokines IL-6, IL-8, IL-16, while IL-12 was significantly decreased. Otherwise there were modest changes in the systemic cytokine kinetics and no significant expression of anti-inflammatory cytokines.

MeSH terms

  • Aged
  • Analysis of Variance
  • Arthroplasty, Replacement, Hip / methods*
  • Cytokines / blood*
  • Female
  • Humans
  • Inflammation Mediators / blood*
  • Interleukin 1 Receptor Antagonist Protein / blood
  • Interleukin-12 / blood
  • Interleukin-16 / blood
  • Interleukin-6 / blood
  • Interleukin-8 / blood
  • Male
  • Middle Aged
  • Postoperative Period*
  • Receptors, Interleukin-2 / blood

Substances

  • Cytokines
  • Inflammation Mediators
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-16
  • Interleukin-6
  • Interleukin-8
  • Receptors, Interleukin-2
  • Interleukin-12