Simvastatin coating of TiO₂ scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells

Biochem Biophys Res Commun. 2014 Apr 25;447(1):139-44. doi: 10.1016/j.bbrc.2014.03.133. Epub 2014 Apr 2.

Abstract

Bone tissue engineering requires an osteoconductive scaffold, multipotent cells with regenerative capacity and bioactive molecules. In this study we investigated the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) on titanium dioxide (TiO2) scaffold coated with alginate hydrogel containing various concentrations of simvastatin (SIM). The mRNA expression of osteoblast-related genes such as collagen type I alpha 1 (COL1A1), alkaline phosphatase (ALPL), osteopontin (SPP1), osteocalcin (BGLAP) and vascular endothelial growth factor A (VEGFA) was enhanced in hAD-MSCs cultured on scaffolds with SIM in comparison to scaffolds without SIM. Furthermore, the secretion of osteoprotegerin (OPG), vascular endothelial growth factor A (VEGFA), osteopontin (OPN) and osteocalcin (OC) to the cell culture medium was higher from hAD-MSCs cultured on scaffolds with SIM compared to scaffolds without SIM. The TiO2 scaffold coated with alginate hydrogel containing SIM promote osteogenic differentiation of hAD-MSCs in vitro, and demonstrate feasibility as scaffold for hAD-MSC based bone tissue engineering.

Keywords: Alginate hydrogel; Human adipose tissue-derived mesenchymal stem cells; Simvastatin; TiO(2) scaffold.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Adult
  • Cell Differentiation / drug effects*
  • Cell Survival
  • Collagen Type I, alpha 1 Chain
  • Female
  • Humans
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / physiology*
  • Middle Aged
  • Osteocalcin / metabolism
  • Osteogenesis*
  • Osteopontin / metabolism
  • Osteoprotegerin / metabolism
  • RNA, Messenger / metabolism
  • Simvastatin / pharmacology*
  • Tissue Engineering / methods
  • Tissue Scaffolds*
  • Titanium
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • COL1A1 protein, human
  • Collagen Type I, alpha 1 Chain
  • Osteoprotegerin
  • RNA, Messenger
  • Vascular Endothelial Growth Factor A
  • Osteocalcin
  • Osteopontin
  • titanium dioxide
  • Simvastatin
  • Titanium