Abstract
Human Hikeshi (HsHikeshi) is a nuclear import carrier for Hsp70s and is required for cell survival after heat shock. The Hikeshi homolog in Schizosaccharomyces pombe (SpHikeshi/Opi10) localizes to the nuclear rim, interacts with the Hsp70 homolog Ssa2, and mediates its nuclear import in a reconstituted mammalian nuclear transport system. However, SpHikeshi/Opi10 is not required for heat stress response and survival after heat stress. Instead, SpHikeshi/Opi10 is required for the normal expression of stress response genes under optimal conditions and for cell growth during glucose deprivation. Here, the functions of SpHikeshi/Opi10 are discussed and compared to the functions of HsHikeshi.
Keywords:
Heat shock; Hikeshi; Hsp70; Nuclear transport; Opi10; Schizosaccharomyces pombe.
Copyright © 2014 Federation of European Biochemical Societies. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus
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Amino Acid Sequence
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Nucleus / metabolism
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Gene Expression Regulation, Fungal
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HSP70 Heat-Shock Proteins / genetics
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HSP70 Heat-Shock Proteins / metabolism
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HeLa Cells
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Heat-Shock Response*
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Humans
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Microscopy, Fluorescence
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Molecular Sequence Data
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Mutation
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Oligonucleotide Array Sequence Analysis
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Protein Binding
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Schizosaccharomyces / genetics
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Schizosaccharomyces / growth & development
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Schizosaccharomyces / metabolism*
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Schizosaccharomyces pombe Proteins / genetics
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Schizosaccharomyces pombe Proteins / metabolism*
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Sequence Homology, Amino Acid
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Temperature
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Transcriptome
Substances
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Carrier Proteins
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HSP70 Heat-Shock Proteins
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Luminescent Proteins
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Opi10 protein, S pombe
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Schizosaccharomyces pombe Proteins
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hikeshi protein, human