This study presents an improved duplex quantitative polymerase chain reaction (qPCR) method using the master sex-determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.
Keywords: aquaculture; fish ecology; molecular diagnostic; molecular sexing; sdY gene; species substitution.
© 2014 The Fisheries Society of the British Isles.