Novel cationic polyene glycol phospholipids as DNA transfer reagents--lack of a structure-activity relationship due to uncontrolled self-assembling processes

Chem Phys Lipids. 2014 Oct:183:117-36. doi: 10.1016/j.chemphyslip.2014.04.006. Epub 2014 May 6.

Abstract

Cationic glycol phospholipids were synthesized introducing chromophoric, rigid polyenoic C20:5 and C30:9 chains next to saturated flexible alkyl chains of variable lengths C6-20:0. Surface properties and liposome formation of the amphiphilic compounds were determined, the properties of liposome/DNA complexes (lipoplexes) were established using three formulations (no co-lipid, DOPE as a co-lipid, or cholesterol as a co-lipid), and the microstructure of the best transfecting compounds inspected using small angle X-ray diffraction to explore details of the partially ordered structures of the systems that constitute the series. Transfection and cytotoxicity of the lipoplexes were evaluated by DNA delivery to Chinese hamster ovary (CHO-K1) cells using the cationic glycerol phospholipid 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (EPC) as a reference compound. The uncontrollable self-association of the molecules in water resulted in aggregates and liposomes of quite different sizes without a structure-property relationship. Likewise, adding DNA to the liposomes gave rise to unpredictable sized lipoplexes, which, again, transfected without a structure-activity relationship. Nevertheless, one compound among the novel lipids (C30:9 chain paired with a C20:0 chain) exhibited comparable transfection efficiency and toxicity to the control cationic lipid EPC. Thus, the presence of a rigid polyene chain in this best performing achiral glycol lipid did not have an influence on transfection compared with the chiral glycerolipid reference ethyl phosphocholine EPC with two flexible saturated C14 chains.

Keywords: Aggregation; Amphiphiles; Carotenoids; Cations; DNA; Lipids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cations
  • Cricetinae
  • Cricetulus
  • Crystallization / methods
  • DNA / chemistry*
  • DNA / genetics*
  • Glycols / chemistry*
  • Indicators and Reagents / chemistry
  • Liposomes / chemical synthesis*
  • Polyenes / chemistry*
  • Structure-Activity Relationship
  • Surface-Active Agents / chemistry
  • Transfection / methods*

Substances

  • Cations
  • Glycols
  • Indicators and Reagents
  • Liposomes
  • Polyenes
  • Surface-Active Agents
  • DNA