Qualitative and quantitative detection of methylation at CpG sites using the fluorescein-dGTP incorporated asymmetric PCR assay strategy

Chem Commun (Camb). 2014 Jun 25;50(50):6653-5. doi: 10.1039/c4cc01933d.

Abstract

The methylation status of each CpG site can be monitored by Fl-dGTP incorporated asymmetric PCR assay. The ability of quantitative detection makes it a good choice for detecting partial methylation at CpG sites compared with others. And the monitoring is not limited to sites within PCR primers or restriction enzyme-recognition sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Assay
  • Cadherins / genetics*
  • CpG Islands / genetics*
  • DNA Methylation*
  • DNA Primers
  • Deoxyguanine Nucleotides / chemistry*
  • Fluorescein / chemistry*
  • Fluorescence
  • Humans
  • Neoplasms / genetics*
  • Polymerase Chain Reaction / methods*
  • Promoter Regions, Genetic / genetics
  • Tumor Cells, Cultured

Substances

  • Cadherins
  • DNA Primers
  • Deoxyguanine Nucleotides
  • deoxyguanosine triphosphate
  • Fluorescein