Prevalence and characterization of enterovirus infections among pediatric patients with hand foot mouth disease, herpangina and influenza like illness in Thailand, 2012

Jiratchaya Puenpa; John Mauleekoonphairoj; Piyada Linsuwanon; Kamol Suwannakarn; Thaweesak Chieochansin; Sumeth Korkong; Apiradee Theamboonlers; Yong Poovorawan

doi:10.1371/journal.pone.0098888

Prevalence and characterization of enterovirus infections among pediatric patients with hand foot mouth disease, herpangina and influenza like illness in Thailand, 2012

PLoS One. 2014 Jun 2;9(6):e98888. doi: 10.1371/journal.pone.0098888. eCollection 2014.

Authors

Jiratchaya Puenpa¹, John Mauleekoonphairoj¹, Piyada Linsuwanon¹, Kamol Suwannakarn¹, Thaweesak Chieochansin¹, Sumeth Korkong¹, Apiradee Theamboonlers¹, Yong Poovorawan¹

Affiliation

¹ Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Abstract

Hand, foot, and mouth disease (HFMD) and herpangina are common infectious diseases caused by several genotypes of human enterovirus species A and frequently occurring in young children. This study was aimed at analyzing enteroviruses from patients with these diseases in Thailand in 2012. Detection and genotype determination of enteroviruses were accomplished by reverse transcription-polymerase chain reaction and sequencing of the VP1 region. Enterovirus-positive samples were differentiated into 17 genotypes (coxsackievirus A4 (CAV4), A5, A6, A8, A9, A10, A12, A16, A21, B1, B2, B4, B5, echovirus 7, 16, 25 and Enterovirus 71). The result showed CAV6 (33.5%), followed by CAV16 (9.4%) and EV71 (8.8%) as the most frequent genotypes in HFMD, CAV8 (19.3%) in herpangina and CAV6 (1.5%) in influenza like illness. Enterovirus infections were most prevalent during July with 34.4% in HFMD, 39.8% in herpangina and 1.6% in ILI. The higher enterovirus infection associated with HFMD and herpangina occurred in infants over one year-old. This represents the first report describing the circulation of multiple enteroviruses in Thailand.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Child
Child, Preschool
Enterovirus / classification
Enterovirus / genetics*
Enterovirus / isolation & purification
Enterovirus Infections / epidemiology
Enterovirus Infections / virology
Female
Genotype
Hand, Foot and Mouth Disease / epidemiology*
Hand, Foot and Mouth Disease / virology*
Herpangina / epidemiology*
Herpangina / virology*
Humans
Infant
Infant, Newborn
Influenza, Human / epidemiology
Influenza, Human / virology
Male
Prevalence
Thailand

Grants and funding

This study was supported by the National Research Council of Thailand; The Higher Education Research Promotion and National Research University Project of Thailand Office of the Higher Education Commission (HR1155A); the Center of Excellence in Clinical Virology, Chulalongkorn University, Centenary Academic Development Project, Integrated Innovation Academic Center; Chulalongkorn University Centenary Academic Development Project (CU56-HR01); the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University (RES560530093); the Outstanding Professor of the Thailand Research Fund (DPG5480002); King Chulalongkorn Memorial Hospital Thai Red Cross Society; and the RGJ PhD program (PHD/0087/2554) and (PHD/0083/2552). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.