Characterisation and immunomodulating activities of exo-polysaccharides from submerged cultivation of Hypsizigus marmoreus

Bing-Zhao Zhang; Kari T Inngjerdingen; Yuan-Feng Zou; Frode Rise; Terje E Michaelsen; Pei-Sheng Yan; Berit S Paulsen

doi:10.1016/j.foodchem.2014.04.092

Characterisation and immunomodulating activities of exo-polysaccharides from submerged cultivation of Hypsizigus marmoreus

Food Chem. 2014 Nov 15:163:120-8. doi: 10.1016/j.foodchem.2014.04.092. Epub 2014 May 4.

Authors

Bing-Zhao Zhang¹, Kari T Inngjerdingen², Yuan-Feng Zou², Frode Rise³, Terje E Michaelsen⁴, Pei-Sheng Yan⁵, Berit S Paulsen²

Affiliations

¹ School of Marine Science and Technology, Harbin Institute of Technology, Weihai Campus, 264209 Weihai, China; Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway; Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, 511458 Nansha, Guangzhou, China.
² Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
³ Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway.
⁴ Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway; Department of Bacteriology and Immunology, Norwegian Institute of Public Health, P.O. Box 4404, Nydalen, 0403 Oslo, Norway.
⁵ School of Marine Science and Technology, Harbin Institute of Technology, Weihai Campus, 264209 Weihai, China. Electronic address: psyan6@hotmail.com.

PMID: 24912706
DOI: 10.1016/j.foodchem.2014.04.092

Abstract

Exo-polysaccharides were purified and characterized from the fermentation broth of Hypsizigus marmoreus, a popular edible mushroom consumed in Asia. Among them, B-I-I and B-II-I exhibited potent complement fixating activity, meanwhile, B-N-I, B-I-I, B-II-I and B-II-II exhibited significant macrophage stimulating activity. Molecular weights of the four exo-polysaccharides were determined to be 6.3, 120, 150 and 11 kDa respectively. Molecular characterisation showed that B-N-I is basically an α-1→4 glucan, with branches on C6; B-I-I is a heavily branched α-mannan with 1→2 linked main chain. B-II-I and B-II-II, have a backbone of rhamno-galacturonan with 1→2 linked l-rhamnose interspersed with 1→4 linked galacturonic acid. Structure-activity relationship analysis indicated that monosaccharide compositions, molecular weight, certain structural units (rhamno-galacturonan type I and arabinogalactan type II) are the principal factors responsible for potent complement fixating and macrophage-stimulating activities. Their immunomodulating activities may, at least partly, explain the health benefits of the mushroom.

Keywords: Complement fixating; Hypsizigus marmoreus; Macrophage stimulating; Molecular structures; Polysaccharides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agaricales / chemistry*
Agaricales / growth & development
Immunologic Factors / pharmacology*
Macrophages / metabolism
Molecular Weight
Monosaccharides / analysis
Nitric Oxide / biosynthesis
Polysaccharides / analysis
Polysaccharides / isolation & purification
Polysaccharides / pharmacology*
Structure-Activity Relationship

Substances

Immunologic Factors
Monosaccharides
Polysaccharides
Nitric Oxide