Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones

Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):730-3. doi: 10.1107/S2053230X14008516. Epub 2014 May 10.

Abstract

CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6-yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P2₁, with unit-cell parameters a=70.0, b=104.8, c=73.5 Å, β=114.4°.

Keywords: Aspergillus oryzae; CsyB; chalcone synthase; csypyrone B1; polyketide synthase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus oryzae / enzymology*
  • Base Sequence
  • Crystallization
  • DNA Primers
  • Escherichia coli / genetics
  • Polyketide Synthases / chemistry
  • Polyketide Synthases / metabolism*
  • Pyrones / metabolism*
  • Recombinant Proteins / genetics

Substances

  • DNA Primers
  • Pyrones
  • Recombinant Proteins
  • Polyketide Synthases