Crystallization and preliminary X-ray crystallographic studies of the ArsI C-As lyase from Thermomonospora curvata

Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):761-4. doi: 10.1107/S2053230X14008814. Epub 2014 May 10.

Abstract

Arsenic is a ubiquitous and carcinogenic environmental element that enters the biosphere primarily from geochemical sources, but also through anthropogenic activities. Microorganisms play an important role in the arsenic biogeochemical cycle by biotransformation of inorganic arsenic into organic arsenicals and vice versa. ArsI is a microbial nonheme ferrous-dependent dioxygenase that transforms toxic methylarsonous acid to the less toxic inorganic arsenite by C-As bond cleavage. An ArsI ortholog from the thermophilic bacterium Thermomonospora curvata was expressed, purified and crystallized. The crystals diffracted to 1.46 Å resolution and belonged to space group P4₃2₁2 or its enantiomer P4₁2₁2, with unit-cell parameters a=b=42.2, c=118.5 Å.

Keywords: ArsI; Thermomonospora curvata; arsenic.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Actinomycetales / enzymology*
  • Base Sequence
  • Crystallization
  • Crystallography, X-Ray
  • DNA Primers
  • Lyases / chemistry*

Substances

  • DNA Primers
  • Lyases