Chiral separation of 12 cathinone analogs by cyclodextrin-assisted capillary electrophoresis with UV and mass spectrometry detection

Electrophoresis. 2014 Nov;35(21-22):3231-41. doi: 10.1002/elps.201400077. Epub 2014 Jun 20.

Abstract

In this study, a rapid chiral separation of 12 cathinones analogs has been developed and validated using cyclodextrin-assisted CE with UV and TOF-MS detection. Optimum separation was obtained on a 57.5 cm × 50 μm capillary using a buffer system consisting of 10 mM β-cyclodextrin (β-CD) in a 100 mM phosphate buffer for CE-UV, and 0.6% v/v highly sulfated-γ-cyclodextrin (HS-γ-CD) in a 50 mM phosphate buffer for CE-MS. In the CE-MS experiment, a partial filling technique was employed to ensure that a minimum amount of cyclodextrin entered the mass spectrometer. All analytes were separated within 18 min in the CE-UV separation and identified by TOF-MS. Ten compounds were enantiomerically separated using β-CD in the UV mode and an additional two more were enantiomerically separated using HS-γ-CD in the MS mode. Detection limits down to 1.0 ng/mL were obtained. The method was then applied to examine seized drugs.

Keywords: CE; Cathinone analogs; Chiral separation; Cyclodextrin; MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / analysis
  • Alkaloids / chemistry*
  • Alkaloids / isolation & purification*
  • Designer Drugs / analysis
  • Designer Drugs / chemistry
  • Electrophoresis, Capillary / methods*
  • Limit of Detection
  • Linear Models
  • Mass Spectrometry / methods*
  • Reproducibility of Results
  • Spectrophotometry, Ultraviolet
  • Stereoisomerism
  • beta-Cyclodextrins / chemistry*

Substances

  • Alkaloids
  • Designer Drugs
  • beta-Cyclodextrins
  • cathinone