Sensitive voltammetric sensor based on isopropanol-Nafion-PSS-GR nanocomposite modified glassy carbon electrode for determination of clenbuterol in pork

Ling Wang; Ran Yang; Jing Chen; Jianjun Li; Lingbo Qu; Peter de B Harrington

doi:10.1016/j.foodchem.2014.04.052

Sensitive voltammetric sensor based on isopropanol-Nafion-PSS-GR nanocomposite modified glassy carbon electrode for determination of clenbuterol in pork

Food Chem. 2014 Dec 1:164:113-8. doi: 10.1016/j.foodchem.2014.04.052. Epub 2014 Apr 30.

Authors

Ling Wang¹, Ran Yang², Jing Chen³, Jianjun Li³, Lingbo Qu⁴, Peter de B Harrington⁵

Affiliations

¹ The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; Department of Chemistry, Zhengzhou Normal University, Zhengzhou 450001, PR China.
² The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: yangran@zzu.edu.cn.
³ The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
⁴ The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; School of Chemistry & Chemical Engineering, Henan University of Technology, Zhengzhou 450001, PR China. Electronic address: qulingbo@zzu.edu.cn.
⁵ Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, OHIO University, Athens, OH 45701-2979, USA.

PMID: 24996313
DOI: 10.1016/j.foodchem.2014.04.052

Abstract

In the present study, poly(sodium 4-styrenesulfonate) (PSS) functionalized graphene (GR) was synthesised via a simple one-step chemical reduction of exfoliated graphite oxides in the presence of PSS. Characterisation of as-made nanocomposite using Fourier transform infrared spectroscopy (FT-IR) and ultraviolet and visible spectroscopy (UV-vis) clearly demonstrate the successful attachment of PSS to graphene sheets. A novel clenbuterol (CLB) electrochemical sensor was fabricated based on isopropanol-Nafion-PSS-GR composite film modified glassy carbon electrode. In the Britton-Robinson buffer (pH 1.2), the sensor exhibited superior electrocatalytic activity towards the oxidation of CLB. Applying linear sweep voltammetry, a good linear relationship of the oxidation peak current with respect to concentrations of CLB cross the range of 7.5 × 10(-8)-2.5 × 10(-5)mol L(-1) and a detection limit of 2.2 × 10(-8) mol L(-1) were achieved. The proposed method was successfully applied for the determination of CLB in pork.

Keywords: Clenbuterol; Electrochemical sensor; Functionalized graphene; Poly(sodium 4-styrenesulfonate).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

2-Propanol
Adrenergic beta-Agonists / analysis*
Animals
Carbon / chemistry
Clenbuterol / analysis*
Electrodes*
Fluorocarbon Polymers
Food Contamination / analysis*
Graphite / chemistry
Limit of Detection
Meat / analysis*
Nanocomposites*
Oxidation-Reduction
Oxides / chemistry
Phosphates
Polymers
Spectroscopy, Fourier Transform Infrared
Sulfonic Acids
Swine

Substances

Adrenergic beta-Agonists
Fluorocarbon Polymers
Oxides
Phosphates
Polymers
Sulfonic Acids
perfluorosulfonic acid
Carbon
Graphite
2-Propanol
sodium polymetaphosphate
Clenbuterol
styrenesulfonic acid polymer