Association of low numbers of CD206-positive cells with loss of ICC in the gastric body of patients with diabetic gastroparesis

Neurogastroenterol Motil. 2014 Sep;26(9):1275-84. doi: 10.1111/nmo.12389. Epub 2014 Jul 13.

Abstract

Background: There is increasing evidence for specific cellular changes in the stomach of patients with diabetic (DG) and idiopathic (IG) gastroparesis. The most significant findings are loss of interstitial cells of Cajal (ICC), neuronal abnormalities, and an immune cellular infiltrate. Studies done in diabetic mice have shown a cytoprotective effect of CD206+ M2 macrophages. To quantify overall immune cellular infiltrate, identify macrophage populations, and quantify CD206+ and iNOS+ cells. To investigate associations between cellular phenotypes and ICC.

Methods: Full thickness gastric body biopsies were obtained from non-diabetic controls (C), diabetic controls (DC), DG, and IG patients. Sections were labeled for CD45, CD206, Kit, iNOS, and putative human macrophage markers (HAM56, CD68, and EMR1). Immunoreactive cells were quantified from the circular muscle layer.

Key results: Significantly fewer ICC were detected in DG and IG tissues, but there were no differences in the numbers of cells immunoreactive for other markers between patient groups. There was a significant correlation between the number of CD206+ cells and ICC in DG and DC patients, but not in C and IG and a significant correlation between iNOS+ cells and ICC in the DC group, but not the other groups. CD68 and HAM56 reliably labeled the same cell populations, but EMR1 labeled other cell types.

Conclusions & inferences: Depletion of ICC and correlation with changes in CD206+ cell numbers in DC and DG patients suggests that in humans, like mice, CD206+ macrophages may play a cytoprotective role in diabetes. These findings may lead to novel therapeutic options, targeting alternatively activated macrophages.

Keywords: gastroparesis; interstitial cells of Cajal; macrophages.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Cell Count
  • Diabetes Mellitus, Type 1 / complications*
  • Female
  • Gastroparesis / etiology
  • Gastroparesis / immunology
  • Gastroparesis / pathology*
  • Humans
  • Interstitial Cells of Cajal / pathology*
  • Lectins, C-Type
  • Macrophages / immunology
  • Macrophages / pathology*
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Middle Aged
  • Receptors, Cell Surface
  • Stomach / immunology
  • Stomach / pathology*

Substances

  • Lectins, C-Type
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Receptors, Cell Surface