Distinctly different dynamics and kinetics of two steroid receptors at the same response elements in living cells

PLoS One. 2014 Aug 18;9(8):e105204. doi: 10.1371/journal.pone.0105204. eCollection 2014.

Abstract

Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/2), binding site occupancy and the resulting temporal changes in transcription upon binding of two closely related steroid receptors, the androgen and glucocorticoid receptors (AR and GR), to their common hormone REs (HREs). We show that there are significant differences at all of these levels between AR and GR at the MMTV HRE when activated by their ligands. These data show that two TFs bound at the same RE can have significantly different modes of action that can affect their responses to environmental cues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cell Line
  • Chromatin Immunoprecipitation
  • Fluorescence Recovery After Photobleaching
  • Humans
  • Receptors, Androgen / genetics
  • Receptors, Androgen / metabolism*
  • Receptors, Glucocorticoid / genetics
  • Receptors, Glucocorticoid / metabolism*
  • Response Elements / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Receptors, Androgen
  • Receptors, Glucocorticoid

Grants and funding

This work was supported by funds from the Norwegian Research Council (www.forskningsradet.no), Norwegian Cancer Society (www.kreftforeningen.no), and University of Oslo (www.uio.no). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.