Phenotypic detection of carbapenemase-producing Enterobacteriaceae by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Carba NP test

James Knox; Snehal Jadhav; Danielle Sevior; Alex Agyekum; Margaret Whipp; Lynette Waring; Jonathan Iredell; Enzo Palombo

doi:10.1128/JCM.02121-14

Phenotypic detection of carbapenemase-producing Enterobacteriaceae by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Carba NP test

J Clin Microbiol. 2014 Nov;52(11):4075-7. doi: 10.1128/JCM.02121-14. Epub 2014 Sep 3.

Authors

James Knox¹, Snehal Jadhav², Danielle Sevior³, Alex Agyekum⁴, Margaret Whipp⁵, Lynette Waring⁶, Jonathan Iredell⁷, Enzo Palombo²

Affiliations

¹ Microbiology Department, Melbourne Pathology, Melbourne, Australia jaknox2@yahoo.com.au.
² Department of Chemistry and Biotechnology, Swinburne University of Technology, Melbourne, Australia.
³ bioMérieux Australia Pty Ltd., Sydney, Australia.
⁴ Westmead Millennium and Marie Bashir Institutes, University of Sydney, Sydney, Australia.
⁵ Microbiological Diagnostic Unit, Public Health Laboratory, University of Melbourne, Melbourne, Australia.
⁶ Microbiology Department, Melbourne Pathology, Melbourne, Australia.
⁷ Westmead Millennium and Marie Bashir Institutes, University of Sydney, Sydney, Australia Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, Australia.

Abstract

We compared the diagnostic accuracy of the Carba NP test with that of a straightforward matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for detecting carbapenemase-producing Enterobacteriaceae (CPE). Using PCR as the reference method, both tests demonstrated a sensitivity of 87% and a specificity of 100%. MALDI-TOF MS offers a potential alternative for the rapid detection of CPE in the clinical laboratory setting.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Bacterial Proteins / analysis*
Bacteriological Techniques / methods*
Enterobacteriaceae / enzymology*
Humans
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
beta-Lactamases / analysis*

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase