Identification of novel piRNAs in bladder cancer

Haiyan Chu; Gaoyun Hui; Lin Yuan; Danni Shi; Yubang Wang; Mulong Du; Dongyan Zhong; Lan Ma; Na Tong; Chao Qin; Changjun Yin; Zhengdong Zhang; Meilin Wang

doi:10.1016/j.canlet.2014.10.004

Identification of novel piRNAs in bladder cancer

Cancer Lett. 2015 Jan 28;356(2 Pt B):561-7. doi: 10.1016/j.canlet.2014.10.004. Epub 2014 Oct 8.

Authors

Haiyan Chu¹, Gaoyun Hui¹, Lin Yuan², Danni Shi¹, Yubang Wang³, Mulong Du¹, Dongyan Zhong⁴, Lan Ma¹, Na Tong¹, Chao Qin⁵, Changjun Yin⁵, Zhengdong Zhang⁶, Meilin Wang⁷

Affiliations

¹ State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China; Department of Environmental Genomics, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
² Department of Urology, Jiangsu Province Hospital of TCM, Nanjing, China.
³ Safety Assessment and Research Center for Drug, Pesticide and Veterinary Drug of Jiangsu Province, School of Public Health, Nanjing Medical University, Nanjing, China.
⁴ Department of Environmental Genomics, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China.
⁵ Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
⁶ State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China; Department of Environmental Genomics, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China. Electronic address: drzdzhang@gmail.com.
⁷ State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China; Department of Environmental Genomics, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, China. Electronic address: mwang@njmu.edu.cn.

PMID: 25305452
DOI: 10.1016/j.canlet.2014.10.004

Abstract

PIWI-interacting RNAs (piRNAs) are a newly identified class of small non-coding RNAs that can play important roles in germline development and carcinogenesis. In this study, piRNA microarrays were used to investigate global piRNA expression in three bladder cancer tissues and their adjacent normal tissues. Using the 3' untranslated region (UTR) sequence complementarily method, we predicted the target gene of piRNA. Our results showed that the expression levels of 106 piRNAs were up-regulated and 91 were down-regulated in bladder cancer tissues, among which the fold-change of down-regulated piRNA DQ594040 associated with bladder cancer (piRABC) was the highest piRNA. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to confirm piRABC expression in paired bladder cancer tissues and their adjacent normal tissues (n = 25). Over-expression of piRABC can inhibit bladder cancer cell proliferation, colony formation, and promote cell apoptosis (all P < 0.05). Luciferase reporter gene assays indicated that piRABC could increase the luciferase activity of TNFSF4. Western blotting analyses and ELISA assays also confirmed that the expression of TNFSF4 protein was up-regulated in control subjects compared with bladder cancer subjects. In conclusion, piRABC plays a crucial role in the development of bladder cancer.

Keywords: Biomarkers; Bladder cancer; piRNA profiles.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis
Blotting, Western
Cell Proliferation*
Enzyme-Linked Immunosorbent Assay
Humans
RNA, Messenger / genetics
RNA, Small Interfering / genetics*
RNA, Small Interfering / metabolism
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Urinary Bladder / metabolism*
Urinary Bladder Neoplasms / genetics*
Urinary Bladder Neoplasms / pathology

Substances

RNA, Messenger
RNA, Small Interfering