Abstract
Although siRNA duplexes are widely used for gene silencing, several unwanted effects such as activation of innate immunity and off-target gene silencing can limit their therapeutic use. Off-targeting can be identified for both the sense and antisense siRNA strands. Some avenues of obstructing the incorporation of the sense strand into the RNA-induced silencing complex (RISC) are currently being pursued. Herein, a biotin group at the 5'-end of the sense strand was used to inhibit its incorporation into the RISC complex. In contrast to chemical modifications, biotin is a naturally occurring compound and its presence in siRNA sequences will not induce side effects.
MeSH terms
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Biotin / metabolism*
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Biotinylation
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Cell Line, Tumor
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Electroporation
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Galectins / antagonists & inhibitors
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Galectins / genetics
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Galectins / metabolism
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Gene Silencing*
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Humans
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Inhibitor of Apoptosis Proteins / antagonists & inhibitors
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Inhibitor of Apoptosis Proteins / genetics
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Inhibitor of Apoptosis Proteins / metabolism
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Molecular Targeted Therapy / methods*
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Neoplasm Proteins / antagonists & inhibitors*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Phosphorylation
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RNA, Messenger / antagonists & inhibitors*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Interfering / genetics*
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RNA, Small Interfering / metabolism
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Receptor, ErbB-2 / antagonists & inhibitors
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Receptor, ErbB-2 / genetics
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Receptor, ErbB-2 / metabolism
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Survivin
Substances
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BIRC5 protein, human
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Galectins
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Inhibitor of Apoptosis Proteins
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Neoplasm Proteins
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RNA, Messenger
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RNA, Small Interfering
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Survivin
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Biotin
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ERBB2 protein, human
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Receptor, ErbB-2