Solution structure of a cucurbit[8]uril induced compact supramolecular protein dimer

Org Biomol Chem. 2014 Dec 14;12(46):9341-4. doi: 10.1039/c4ob01729c. Epub 2014 Oct 22.

Abstract

Supramolecular assembly of a beta-barrel protein via cucurbit[8]uril results in compact z-shaped protein dimers. SAXS data reveal the formation of a well ordered protein dimer, notwithstanding being connected by a reversible and flexible peptide linker, and highlight the supramolecular induced interplay of the proteins, analogous to covalently linked proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bridged-Ring Compounds / chemistry*
  • Imidazoles / chemistry*
  • Luminescent Proteins / chemistry*
  • Models, Molecular
  • Molecular Weight
  • Protein Multimerization
  • Protein Structure, Secondary
  • Recombinant Fusion Proteins / chemistry*
  • Scattering, Small Angle
  • Solutions
  • X-Ray Diffraction

Substances

  • Bacterial Proteins
  • Bridged-Ring Compounds
  • Imidazoles
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Solutions
  • cucurbit(8)uril
  • yellow fluorescent protein, Bacteria