Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy

Birgit De Smet; Derek S Sarovich; Erin P Price; Mark Mayo; Vanessa Theobald; Chun Kham; Seiha Heng; Phe Thong; Matthew T G Holden; Julian Parkhill; Sharon J Peacock; Brian G Spratt; Jan A Jacobs; Peter Vandamme; Bart J Currie

doi:10.1128/JCM.02574-14

Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy

J Clin Microbiol. 2015 Jan;53(1):323-6. doi: 10.1128/JCM.02574-14. Epub 2014 Nov 12.

Authors

Affiliations

¹ Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
² Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia derek.sarovich@menzies.edu.au.
³ Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.
⁴ Sihanouk Hospital Centre of HOPE, Phnom Penh, Cambodia.
⁵ Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
⁶ Wellcome Trust Sanger Institute, Cambridge, United Kingdom University of St. Andrews, St. Andrews, United Kingdom.
⁷ Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
⁸ Wellcome Trust Sanger Institute, Cambridge, United Kingdom University of Cambridge, Cambridge, United Kingdom.
⁹ Imperial College, London, United Kingdom.
¹⁰ Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium.
¹¹ Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.

Abstract

Burkholderia pseudomallei isolates with shared multilocus sequence types (STs) have not been isolated from different continents. We identified two STs shared between Australia and Cambodia. Whole-genome analysis revealed substantial diversity within STs, correctly identified the Asian or Australian origin, and confirmed that these shared STs were due to homoplasy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Australia
Bacterial Typing Techniques*
Burkholderia pseudomallei / genetics*
Cambodia
Evolution, Molecular
Genome, Bacterial / genetics
Humans
Melioidosis / microbiology*
Multilocus Sequence Typing*
Phylogeny

Abstract

Publication types

MeSH terms

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