Cynoglossus zanzibarensis (Cynoglossidae, Soleoidei) is characterized by both eyes on the left side of the body. Here we report the mitogenome of this tonguesole for the first time, which is 16,569 bp in length, with gene order reorganized. The tRNA-Gln gene is translocated from the light strand (L-strand) to the heavy strand (H-strand), accompanied by shuffling of tRNA-Ile and tRNA-Met genes with a 160-bp intergenic spacer between tRNA-Gln and tRNA-Ile. The putative control region is translocated downstream to the place between the ND1 and the tRNA-Gln genes, leaving a 25-bp trace fragment in the original position. The rest of the gene arrangement is identical to that of the typical fish.
Keywords: Mitochondrial DNA; Zanzibar tonguesole; reorganization.