Interchange of L polymerase protein between two strains of viral hemorrhagic septicemia virus (VHSV) genotype IV alters temperature sensitivities in vitro

Virus Res. 2015 Jan 2:195:203-6. doi: 10.1016/j.virusres.2014.10.013. Epub 2014 Oct 18.

Abstract

Viral hemorrhagic septicemia virus (VHSV) has four genotypes (I-IV) and sub-lineages within genotype I and IV. Using a reverse genetics approach, we explored the importance of the L gene for growth characteristics at different temperatures following interchange of the L gene within genotype IV (IVa and IVb) strains. VHSV strains harboring heterologous L gene were recovered and we show that the L gene determines growth characteristics at different temperatures in permissive cell lines.

Keywords: Genotype IV; L protein; Reverse genetics; Temperature sensitivity; Viral hemorrhagic septicemia virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Fishes
  • Genotype
  • Novirhabdovirus / enzymology*
  • Novirhabdovirus / genetics
  • Novirhabdovirus / physiology*
  • Novirhabdovirus / radiation effects
  • RNA-Dependent RNA Polymerase / genetics
  • RNA-Dependent RNA Polymerase / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Reverse Genetics
  • Temperature
  • Virus Replication / radiation effects*

Substances

  • Recombinant Proteins
  • RNA-Dependent RNA Polymerase